High-Performance Liquid 
         Chromatography (HPLC) Systems
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    Calibration.aspx
              Introduction Of HPLC
     • HPLC stands for “High-performance liquid chromatography”(sometimes referred to as 
      High- pressure liquid chromatography).
     • Chromatography  :  physical  method  in  which  separation  of  components  takes  place 
      between two phases
        1. Stationary phase and 
        2. Mobile phase
       Stationary phase : The substance on which adsorption of the analyte (the substance 
       to be separated during chromatography) takes place . It can be a solid, a gel, or a solid 
       liquid combination
       Mobile phase : Solvent which carries the analyte (a liquid or a gas)
     • powerful  tool  in  analysis,  it  yields  high  performance  and  high  speed  compared  to 
      traditional columns chromatography because of the forcibly pumped mobile phase.
     Qualification Approach
             Installation Qualification 
                         (IQ)
     • Verity approved purchase order. 
       • Verity invoice Check manufacturer and supplier.
       • Verity model number and serial number. 
       • Check for any physical damage. 
       • Confirm location and installation requirements per recommendation of 
        manufacturer. 
       • Verify that the required utilities are available. 
       • Installation shall be conducted per instructions provide in the manual.
    Before installation
    • Obtain manufacturer's recommendations for installation site 
     requirements.
    • Check the site for the fulfillment of the manufacturer’s 
     recommendations (utilities such as electricity, and environmental 
     conditions such as humidity and temperature).
    • Allow sufficient shelf space for the equipment, SOPs, operating 
     manuals and software.