Overview
    I.  Exp. 16:  Identification of Unknown 
        Microorganisms
    I.  Exp. 16:  Identification of Unknown      
                 Microorganisms                
     TODAY:
      ◦Interpret Temp.  Study (Exp. 7-Table1)
        Determine Optimum Temp.
        Any contaminants in tubes?
      ◦Observe/Describe Colony Morphology 
       Plate
      ◦Repeat Gram Stain 
        Does it agree with last week’s results?
      ◦If need be, repeat Wet Mount & 
       Simple Stain
      ◦Set up the necessary tests for YOU!
        Incubate at YOUR  Optimum Temp.
    I.  Exp. 16:  Identification of Unknown 
       Microorganisms                 
      Yeast
      ◦Opt. Temp:  5, 25, 37oC 
      ◦Dry,white, crusty growth
      ◦Wet Mount:  large, ovoid, irregular, budding,  
                  Brownian Movement
      ◦Simple Stain: large, ovoid, irregular, budding
      ◦Gram Stain: Same as above, cells 
         stain either purple, pink or both 
      ◦Catalase:  + (bubbles)  
      ◦No other testing necessary
    I.  Exp. 16:  Identification of Unknown 
       Microorganisms                 
      Gram Pos. Cocci:  Staph and Strep
       ◦                       o
         Opt. Temp:  25-37 C 
       ◦Small white creamy colonies
                        Staphylococcus
       ◦Gram Pos. cocci in clusters
       ◦Catalase:  +
       ◦Halophile        
    I.  Exp. 16:  Identification of Unknown 
      Microorganisms                 
                     Streptococcus
      ◦Gram Pos. cocci in chains
      ◦Catalase:  -
      ◦Not a halophile      
     Today:
        1.  Do Catalase test (Exp. 15)
        2.  Inoculate Salt Plate (Staph 110 or MSA)