Background
  8 conventional laboratories serve the whole 
   country needs for viral load with long TAT.
  Logistics of sample transportation are a bottleneck 
   to achieving optimal results TAT.
  Point-of-care (POC) test for CD4 and EID have 
   shown positive impact in patient important 
   outcomes.
  POC for viral load (POCVL) would significantly 
   improve management of HIV+ patients
       m-PIMA HIV-1/2 VL Test 
  Instrument: Scaled up for EID testing at 130 
   health facilities.
  Investigational use only cartridge : Quantitative 
   HIV-1/2 viral load (VL)
  Requires 50µl of plasma sample 
  Test result in less than 70 min
  Kit’s volumetric transfer tool simplifies sampling
                   Methods
    Cross-sectional study in 2 PHCC in Maputo City
    Sample size: 699 (Women PMTCT cascade)
      233 patients for each VL interval (< 1000 cps/ml, 1000-
       10000 cps/ml, >10000 cps/ml)
    1 microtube 0,5mL venous blood mini-centrifuged 
     (not provided by the manufacturer) for POCVL 
     testing 
    6 ml EDTA tube- Plasma conventional testing 
     (CAPCTM Roche)
    1 DBS card (routine VL in Mozambique)
                    Study Sample Flowchart
                             500ul for             Centrifugation          50ul plasma to 
                                                        with                   m-pima 
                             microtube             Mini-centrifuge            cartridge
      1 EDTA tube               DBS                 Molecular 
         of 6 ml             (routine)            Centralized lab           Testing
                                                      of INS
                            Remaining               Molecular           Centrifugation 
                               blood              Centralized lab       and processing
                                                      of INS