Introduction to Chromatography
             Column chromatography and HPLC
                     Pharmaceutical Instrumental Analysis
                          Lina A. Dahabiyeh
   Basic of Chromatography
   Chromatography is a separation technique to seprate mixtures into their
    components in order to identify (qualitative analysis), purify and/or quantify
    (quantitive analysis) the mixture or components.
   Chromatography is the most frequently used analytical technique in
    pharmaceuticalanalysis.
   Chromatography aims to separate a mixture of chemical substances into its
    individual components, so that the individual components can be thoroughly
    analyzed.
   TwoPhases:
      Stationary Phase (stays in a place): Liq or solid
      MobilePhase(flowsthroughcolumnormoveinaplate,carriesanalyte):Liqorgas
    History of Chromatography
   The Russian botanist Mikhail Tswett coined the term
    chromatography in 1906 to describe his experiments in
    separating different colored constituents of leaves through a
    column.
   ”Chromatography”;Khroma(colour)andgraphic(writing)
      Classification of Chromatography
       Based on:
       Mechanism of Separation: Normal or Reversed,   ion-exchange
         chromatography,affinity chromatography
       Shape of the stationary Phase: Column or Planer
       The Physical State of the mobile phase: Liquid chromatography (LC) or 
         Gas chromatography (GC)
       The purpose of separation: Analytical or Preparative