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picture1_Spectrophotometry Slideshare 66726 | Spectrophotometry


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File: Spectrophotometry Slideshare 66726 | Spectrophotometry
spectrophotometry particulary in the visible region of the electromagnetic spectrum is one of the most widely used methods of analysis it is very widely used in clinical chemistry and environmental ...

icon picture PPTX Filetype Power Point PPTX | Posted on 27 Aug 2022 | 3 years ago
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    Spectrophotometry , particulary in the visible region of the electromagnetic 
    spectrum ,is one of the most widely used methods of analysis . It is very widely used 
    in clinical chemistry and environmental laboratories because very substances can be 
     selectively converted to a colored derivative.  The instrumentation is readly 
    available an generally fairly easy to operate.  
       The wave is described either in terms of its wavelength, the distance of one complete cycle , or in 
       terms of the frequency , the number of cycles passing a fixed point per unit time. The reciprocal of 
       the wavelength  is called the wavenumber.
       The relation between the wavelength and frequency is
                                     Spectral changes can be classed as follows :
                                     1.   Bathochromic  shift  :  absorption  maximum  shifted  to 
                                          longer  wavelength.
                                     2.   Hipsochromic shift : absorption maximum shifted to longer 
                                           wavelength.
                                     3. Hyperchromism effect  : an increase in molar absorptivity
                                     4. Hypochromism effect  : a descrease in molar absorptivity
                                      
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...Spectrophotometry particulary in the visible region of electromagnetic spectrum is one most widely used methods analysis it very clinical chemistry and environmental laboratories because substances can be selectively converted to a colored derivative instrumentation readly available an generally fairly easy operate wave described either terms its wavelength distance complete cycle or frequency number cycles passing fixed point per unit time reciprocal called wavenumber relation between spectral changes classed as follows bathochromic shift absorption maximum shifted longer hipsochromic hyperchromism effect increase molar absorptivity hypochromism descrease...

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