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picture1_Spectrophotometry Slideshare 68397 | 1635236765721205


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File: Spectrophotometry Slideshare 68397 | 1635236765721205
calibration curve calibration curve also called standard curve is a general method for determining the concentration of a substance in unknown sample by comparing the unknown to a set of ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago
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                 CALIBRATION CURVE
          • Calibration curve, also called standard curve, is 
           a general method for determining the 
           concentration of a substance in unknown 
           sample by comparing the unknown to a set of 
           standard samples of known concentration.
                 CALIBRATION CURVE
          • The curve prepared from a series of dilutions 
           from standard solutions of a certain substance 
          • Used as a reference curve to obtain the 
           concentration of unknown samples of that 
           substance.
               SPECTROPHOTOMETRY 
          • Spectrophotometry is a method to measure 
           how much a chemical substance absorbs light, 
           by measuring the intensity of light, as a beam 
           of light passes through sample solution. 
          • The basic principle behind this method is that : 
           “Each compound absorbs or transmits light 
           over a certain range of wavelength”
               SPECTROPHOTOMETRY
         • The light absorbed by the sample is directly 
          proportional to the concentration of sample in 
          the solution.  As concentration increases , 
          absorption increases exponentially.
                    HOW IT WORKS ?
           •  When light goes through the substance some 
            of it is reflected & the rest is partially absorbed 
            and partially transmitted. 
           • Spectrophotometric method of analysis is 
            usually concerned with measurement of the 
            amount of light absorbed.
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...Calibration curve also called standard is a general method for determining the concentration of substance in unknown sample by comparing to set samples known prepared from series dilutions solutions certain used as reference obtain that spectrophotometry measure how much chemical absorbs light measuring intensity beam passes through solution basic principle behind this each compound or transmits over range wavelength absorbed directly proportional increases absorption exponentially it works when goes some reflected rest partially and transmitted spectrophotometric analysis usually concerned with measurement amount...

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