323x Filetype PPTX File size 1.82 MB Source: nyfoodsafety.cals.cornell.edu
Objectives
Microbial genomes
Common isolate identification techniques
using molecular biology
Whole genome sequencing (WGS)
Example of WGS for outbreak investigation
Questions
Microbial Genomes
Genome size varies from 4.56 to 5.70 Mb
How big is 5 Mb???
Harry Potter Story
Long story, some big books!
1,084,440 words in all seven books
Average word length ~5 letters
~5,422,200 letters total in box set
E.coli genomes range from 4.56 to 5.70 Mb
A single E. coli genome @ 1 box set
Single human genome @ 1,000 box sets!!!
PFGE (Pulsed Field Gel
Electrophoresis)
What do these bands really mean???
PFGE (Pulsed Field Gel
Electrophoresis)
1 site 2 sites 3 sites 2 sites 4 sites
5 Mb 5 Mb 5 Mb 5 Mb 5 Mb
b
M 5Mb
n
i
e
z
i
s
e
m 1Mb
o
n
e
G 0.5Mb
Restriction enzyme site
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