202x Filetype PPTX File size 0.70 MB Source: pscp.org.pk
Q:1: The analytical technique in which optics (Photometry) is NOT used in any form: a. Atomic Absorption b. HPLC c. POCT for glucose d. Radioimmunoassay e. Real Time PCR Best answer: d. Radioimmunoassay Optical Techniques used in Various Instruments Atomic Absorption Photometry: Primarily a Photometric Technique HPLC: Photometry used in detection in many types of HPLC Glucometers: Reflectance technique Real Time PCR: Fluorometry in detection Q: 2. A Chemical Pathology autoanalyser has rejected the blank cuvettes before the start of analysis with the message “Cuvette Check Fail” (Dirty Cuvettes). Which of the following phenomena is mainly prevented by this function of autoanalyser: a. Absorbance of light b. Emission of light c. Reflectance of light d. Scattering of Light e. Transmission of light Best answer: d.Scattering of Light Q:3: Which of the following modifications makes a Liquid Chromatography a High Performance Liquid Chromatography (HPLC): a. Fixed loop injector b. Increasing volatility by sample derivatization c. Length of columns from 50 to 250 nm d. Small-diameter particles as stationary phase e. UV lamp detectors of 190 to 400 nm. Best answer: d. Small-diameter particles as stationary phase Q:4: It is reported that the sensitivity of an Atomic Absorption method is 100 times higher than Flame Emission. The reason for this very high sensitivity is: a. Drastic reduction in background noise b. Most of the ground state atoms absorb light. c. Sample derivatization before analysis d. Use of a hollow cathode lamp made of the material to be analysed. e. Very high temperature is used for excitation of atoms. Best answer: b. Most of the ground state atoms absorb light.
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