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2
Introduction
Spectroscopy is the study of the interaction between matter and electro-
magnetic radiation. Historically, spectroscopy originated through the study
of visible light dispersed according to its wavelength, by a prism. Later the
concept was expanded greatly to comprise any interaction with radiative
energy as a function of its wavelength or frequency. Spectroscopic data is
often represented by a spectrum, a plot of the response of interest as a
function of wavelength or frequency.
• Spectrum (Latin): ghost
• Skopos (Greek): watcher
• Spectroscopist = ghost watcher
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© Agilent Technologies, Inc. 2016
3
Table of Contents
Historical Background Key Parameters
• Early History of Optical Spectra • Wavelength and Frequency
• 1666 Observation of Visible Spectrum • Absorption and Emission
• 1802 Fraunhofer Absorption Lines • Light Absorbed vs. Energy Levels
• Kirchhoff & Bunsen’s Emission • Characteristics of Atomic Spectra
Experiment • Absorbance and Transmittance
• Kirchhoff & Bunsen’s Absorption • Absorbance: Concentration
Experiment Relationship
• Beer-Bouguer-Lambert Law
Definitions
• Spectroscopy and Spectrometer
• Electromagnetic Spectrum
• Light
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4
Historical Background
Early History of Optical Spectra
1666 1802 1812 1853 1859 1868 1882
Sir Isaac William Hyde Joseph von August Beer Gustav Anders J. Abney &
Newton Wollaston Fraunhofer recognizes Kirchhoff & Angstrom Festing
discovers identifies dark studies these the Robert measures the obtained
solar lines in solar dark lines relationship Bunsen wavelengths infrared
spectrum spectrum using a between the observe of about absorption
spectroscope absorption of different 1,000 spectra for
light and colors from Fraunhofer over 50
concentration elements lines compounds
heated to
incandescenc
e
ToC
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5
Historical Background
1666 Observation of Visible Spectrum
Sir Isaac Newton`s Experiment
Sir Isaac Newton,1642-1726
English physicist and mathematician
Source: Wikipedia
ToC
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© Agilent Technologies, Inc. 2016
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